[Objective] To establish HPLC analyses method for the β-elemene content in L. camara; and to detect the β-elemene content and distribution law of malignant weed L. camara in Panxi Area of Sichuan Province of China. [Method] HPLC method was used to detect the β-elemene content in L. camara. Eclipse XDB-C18 (4.6 mm × 150 mm, 5 μm) chromatographic column was adopted. The flow rate was 1.0 ml/min; detection wavelength was 210 nm; injection volume was 20 μl; column temperature was 30 ℃; and mobile phase was ethanol-acetonitrile-water (V/V/V, 70∶ 10∶ 20). Ultraviolet ultraviolet spectrophotometer and TLC were both used for the analyses of β-elemene in sample. [Result] β-elemene content in L. camara was 0.007 5-0.120 0 mg/ml by HPLC method, showing good linear relationship (r=0.999 5). And the average recovery rate was 99.3%.There was β-elemene in both the leaves and flowers of L. camara. And β-elemene content was the highest in leaves. β-elemene contents in fresh leaves and natural drying leaves were 0.025% and 0.083%, respectively. There were no significant differences between fresh leaves and natural drying leaves; but the two were significantly higher than that in 60 ℃ drying leaves. [Conclusion] This method was suitable for the content determination of β-elemene in L. camara. It also provided references for the development and utilization of L. camara, and offered new sources for the extraction of L. camara. [Objective] To establish HPLC analysis methods for the β-elemene content in L. camara; and to detect the β-elemene content and distribution law of malignant weed L. camara in Panxi Area of ​​Sichuan Province of China. [Method] HPLC method was used to detect the β-elemene content in L. camara. The Eclipse XDB-C18 (4.6 mm × 150 mm, 5 μm) chromatographic column was adopted. The flow rate was 1.0 ml / min; was 20 μl; column temperature was 30 ° C; and mobile phase was ethanol-acetonitrile-water (V / V, 70:10:20). Ultraviolet ultraviolet spectrophotometer and TLC were both used for the analyzes of β-elemene in sample . [Result] β-elemene content in L. camara was 0.007 5-0.120 0 mg / ml by HPLC method, showing good linear relationship (r = 0.999 5). And the average recovery rate was 99.3%. Where was β-elemene in both the leaves and flowers of L. camara. And β-elemene content was the highest in leaves. β-elemene contents in fresh leaves and There were no significant differences between fresh leaves and natural drying leaves; but the two were significantly higher than that at 60 ° C drying leaves. [Conclusion] This method was suitable for the content determination of β-elemene in L. camara. It also provided references for the development and utilization of L. camara, and offered new sources for the extraction of L. camara.