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以新疆哈密瓜(Cucumis melo L.)幼叶为材料,根据已报道的甜瓜ACC合成酶3(1-aminocy-clopropane-1-carboxylic acid synthase,ACS)基因片段设计特异引物,采用RT-PCR和RACE(Rapid Amplifi-cation cDNA Ends,cDNA末端快速扩增)等技术,克隆得到编码哈密瓜CMe-ACS3基因的全长cDNA序列,长1895bp,编码482个氨基酸,分子量约为54.12kD,等电点8.45,GenBank登录号为FJ383171。利用软件MEGA4构建进化树,发现哈密瓜ACS3基因与黄瓜中的同类基因亲缘关系最近。哈密瓜CMe-ACS3基因的克隆及序列分析为认识此基因在甜瓜中的功能研究奠定基础。
In this study, specific primers were designed according to reported genes of 1-aminocy-clopropane-1-carboxylic acid synthase (ACS) in young leaves of Cucumber (Cucumis melo L.) Xinjiang using RT-PCR and RACE (Rapid Amplification cDNA Ends, rapid amplification of cDNA ends) and other technology, the full length cDNA sequence of CMe-ACS3 gene encoding Hami melon was cloned, encoding a polypeptide of 482 amino acids with a length of 1895 bp, a molecular weight of about 54.12 kD and an isoelectric point of 8.45, GenBank accession number is FJ383171. Using software MEGA4 to construct phylogenetic tree, it was found that the cantaloupe ACS3 gene has the closest genetic relationship with the same gene in cucumber. The cloning and sequence analysis of CMe-ACS3 gene in Hami melon lay the foundation for understanding the function of this gene in melon.