目的：总结冰冻切片诊断卵巢肿瘤的体会。方法：回顾性分析８５９例卵巢肿瘤冰冻切片诊断资料。结果：利用冰冻切片诊断卵巢肿瘤，确诊率９４．８８％，未能确诊率０．５８％，误诊率４．５４％。误诊３９例，多见于交界性粘液囊腺瘤，分化好粘液囊腺癌和转移性粘液腺癌；４例冰冻切片误诊的良性肿瘤包括２例卵巢甲状腺肿和２例粘液囊腺瘤。误诊原因中，取材不当占５３．８８％，读片错误３５．９０％，制片不良１０．２８％。结论：冰冻切片是手术中确定卵巢肿瘤性质的有效方法。提高取材技术、掌握疑难病例大体表现和镜下特征和必要时辅助印片和组化染色，有望提高冰冻切片诊断卵巢肿瘤的准确性 Objective: To summarize the experience of frozen section diagnosis of ovarian tumors. Methods: The diagnostic data of 859 cases of ovarian tumors were retrospectively analyzed. Results: Using frozen section to diagnose ovarian tumors, the diagnosis rate was 94.88%, the rate of undiagnosis was 0.58%, and the misdiagnosis rate was 4.54%. 39 cases were misdiagnosed, and were more common in borderline mucinous cystadenoma, well-differentiated mucinous cystadenocarcinoma and metastatic mucinous adenocarcinoma. Four cases of misdiagnosed benign neoplasms included frozen ovary goiter and 2 mucinous cystadenoma. Of the misdiagnosis reasons, 53.88% were improperly obtained, 35.90% were incorrectly read, and 10.28% were defective. Conclusion: Frozen section is an effective method to determine the nature of ovarian tumors during surgery. Improve the acquisition techniques, master the general performance and microscopic features of intractable cases, and assist printing and histochemical staining when necessary, which may improve the accuracy of frozen section diagnosis of ovarian tumors.