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以抗旱性不同的3个大豆品种(湘豆3号、Lee68和宁镇1号)为试材,研究了不同浓度(5%、10%、15%、20%、25%和30%)的PEG-6000模拟发芽期干旱胁迫对大豆种子发芽、子叶苗膜脂过氧化以及渗透调节物质含量的影响。结果表明:10%以下的PEG-6000能起引发作用,促进大豆种子的萌发,显著提高发芽势和发芽率;随着PEG-6000浓度的升高,大豆种子的发芽明显受抑制,当PEG-6000浓度大于25%时抑制更明显,发芽势、发芽率、发芽指数和简易活力指数显著下降;随着PEG-6000浓度的升高,大豆子叶苗中的丙二醛(MDA)、脯氨酸(Pro)、可溶蛋白和可溶性糖含量显著增加,PEG-6000浓度在15%~25%间增加幅度最大,PEG-6000浓度小于15%或大于25%时增加不明显。综合种子发芽、子叶苗的膜脂过氧化程度以及渗透调节物质含量的变化,可初步确定室内采用PEG-6000浸种法模拟干旱胁迫鉴定大豆发芽期耐旱性的适宜浓度为20%~25%。
Three soybean cultivars with different drought resistance (Xiangdou 3, Lee68 and Ningzhen 1) were used to study the effects of different concentrations (5%, 10%, 15%, 20%, 25% and 30% Effects of PEG-6000 Simulated Germination Stress on Seed Germination, Membrane Lipid Peroxidation and Osmotic Adjustment Substances in Soybean Seedling during Germination. The results showed that PEG-6000 (less than 10%) could induce the germination of soybean seeds and significantly increase the germination energy and germination rate. With the PEG-6000 concentration increasing, the germination of soybean seeds was significantly inhibited. When PEG- 6000 concentration significantly inhibited more obvious when the germination potential, germination rate, germination index and simple vitality index decreased significantly; With the PEG-6000 concentration increased, the soybean seedlings of malondialdehyde (MDA), proline (Pro). The content of soluble protein and soluble sugar increased significantly. The maximum increase of PEG-6000 concentration was between 15% and 25%, but no obvious increase when the concentration of PEG-6000 was less than 15% or more than 25%. Based on the changes of membrane lipid peroxidation and the content of osmotic adjusting substances in seed germination, the optimum concentrations of indoor drought tolerance of 20% ~ 25% were identified by PEG-6000 seed germination simulation to simulate drought stress in soybean germination stage.