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目的:了解CCL4对小鼠机体的影响,建立急性肝损伤动物模型,用于其保肝功能药物的筛选。方法:小鼠按0.1ml/kg.b.w一次性腹腔注射染毒,设空白对照组、溶剂对照组及3个剂量组(浓度为0.12%、0.14%、0.16%),每个浓度按16、18、24、48、72h采血,测定血清天门冬氨酸氨基转移酶(AST)、丙氨酸氨基转移酶(ALT)活力,计算肝脏系数、脾脏系数并进行肝组织病理学检查。结果:与溶剂对照组比较,16h0.12%染毒和18h0.14%染毒只是血清AST升高(P<0.01~0.05),其余浓度对血清AST、ALT的影响(P>0.05);24h不同染毒(0.12%、0.16%)血清AST、ALT升高(P<0.01~0.05),0.14%染毒对血清AST、ALT的影响(P>0.05);48h不同染毒(0.12%、0.14%、0.16%)不同时间(16、18、24、48h)对小鼠肝脏系数的影响(P>0.05);CCL4不同染毒(0.12%、0.14%、0.16%)不同时间(16、18、24h)对小鼠脾脏系数的影响(P>0.05),而不同染毒(0.12%、0.14%、0.16%)48h脾脏系数有所降低(P<0.01~0.05)。0.12%CCL4染毒24h肝组织结构广泛性破坏,肝细胞大量坏死,有炎细胞浸润。结论:0.12%CCL4染毒24h,是较理想的检测及保肝功能保健食品的动物模型。
OBJECTIVE: To understand the effect of CCL4 on the body of mice and to establish an animal model of acute liver injury for the screening of its hepatoprotective drugs. Methods: The mice were injected intraperitoneally with 0.1ml / kg.bw intraperitoneally to establish a blank control group, a solvent control group and three dose groups (0.12%, 0.14% and 0.16% respectively) Blood samples were taken at 18, 24, 48 and 72h. Serum aspartate aminotransferase (AST) and alanine aminotransferase (ALT) activity were measured. Liver coefficient and spleen coefficient were calculated. Liver histopathology was also performed. Results: Compared with the solvent control group, 16h0.12% and 18h0.14% exposure were only elevated serum AST (P <0.01 ~ 0.05) and other concentrations of serum AST and ALT (P> 0.05); 24h (P <0.01 ~ 0.05), and the effect of 0.14% exposure on serum AST and ALT (P> 0.05). The effects of different exposures (0.12%, 0.16% %, 0.16%) at different times (16, 18, 24, 48h) on hepatic coefficient of mice (P> 0.05) (P> 0.05). However, the spleen coefficient decreased 48h after exposure to different doses of (0.12%, 0.14%, 0.16%) (P <0.01 ~ 0.05). 0.12% CCL4 24h liver tissue damage extensive destruction, a large number of liver cell necrosis, inflammatory cell infiltration. Conclusion: 0.12% CCL4 exposure 24h, is an ideal animal model of testing and liver function health food.