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通过对不同动物种属心脏细胞膜上3H-哇巴因特异性结合的分析表明,猫、羊、小牛、人的心脏中只有一个哇巴因结合点,而在大鼠(可能还在豚鼠)的心脏中则有二个结合点。为了研究这一发现的生物学意义,在一定的条件下测定不断增加哇巴因浓度时,心肌Na~+K~+ATP酶的活性、~(86)Rb~+的摄取以及心脏制备收缩力的变化。结果表明,(1)~3H-哇巴团结合在心脏细胞膜上与结合到由电刺激引起兴奋的心脏制备上,具有相同的性质,即有相同的解离常数;(2)在猫心,~3H-哇巴因的结
Analysis of the 3H-ouabain-specific binding on the membranes of heart cells of different species of animals shows that there is only one ouabain binding site in cats, sheep, calves and humans, whereas in rats (and possibly in guinea pigs) There are two binding points in the heart. In order to study the biological significance of this finding, the activity of Na ~ + K ~ + ATPase, the uptake of ~ (86) Rb ~ +, and cardiac contractility were measured under certain conditions with increasing ouabain concentration The change. The results showed that (1) ~ 3H-wah bolus had the same properties, ie, the same dissociation constants, on the membrane of cardiac cells as well as in the preparation of heart bound to excitement caused by electrical stimulation. (2) ~ 3H-ouabain knot