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目的比较Cervista酶切信号扩大法与人乳头瘤病毒(HPV)快速筛查法对高危型人乳头瘤病毒(HRHPV)的检测在宫颈癌早期筛查中的应用价值。方法对新疆巴楚县373例维吾尔族妇女行Cervista HR-HPV检测及HPV快速筛查法检测,并均行阴道镜下宫颈活检。比较两种检测方法的检测结果及一致性。结果在373例标本中,Cervista HR-HPV DNA检测的HPV阳性315例,阳性率为84.5%,HPV快速筛查法检测的HPV阳性为327例,阳性率为87.7%。两种检测方法结果一致者343例,一致率为92.0%,κ值为0.81。两种高危型HPV检测方法中炎症、宫颈上皮内瘤变(CIN)Ⅰ、CINⅡ、CINⅢ、宫颈癌的阳性率均有统计学差异(P均<0.01),随着病理级别增高,HPV感染率呈增高趋势;A9组在CIN及宫颈癌组中较A5/A6组、A7组感染率高。结论两种检测方法均可用于临床检测HR-HPV。A9组对于维吾尔族妇女宫颈病变有很高的预测价值。Cervista HR-HPV DNA检测可作为HPV快速筛查法灵敏的分流方法用于经济欠发达地区的宫颈癌筛查。
Objective To compare the diagnostic value of Cervista digestion signal amplification method and human papillomavirus (HPV) rapid screening method in the early screening of cervical cancer in high-risk human papillomavirus (HRHPV). Methods Cervista HR-HPV test and HPV rapid screening test were performed on 373 Uighur women in Bachu County, Xinjiang Uygur Autonomous Region. Cervical biopsy was performed under colposcopy. The test results and consistency of the two test methods were compared. Results Of the 373 specimens, 315 were positive for HPV detected by Cervista HR-HPV DNA, with a positive rate of 84.5%. The HPV positive rate by HPV rapid screening method was 327, with a positive rate of 87.7%. 343 results were consistent with the two detection methods, the agreement rate was 92.0%, κ value was 0.81. The positive rates of inflammation, CIN Ⅰ, CINⅡ, CINⅢ and cervical cancer in two high-risk HPV test methods were significantly different (all P <0.01). With the increase of pathological grade, the HPV infection rate A9 group in CIN and cervical cancer group than the A5 / A6 group, A7 group infection rate is high. Conclusion Both methods can be used for clinical detection of HR-HPV. The A9 group has a high predictive value for cervical lesions in Uighur women. The Cervista HR-HPV DNA test can be used as a sensitive shunt method for HPV rapid screening for cervical cancer screening in economically underdeveloped areas.