为构建带有碱性磷酸酶活性的双功能基因工程抗体,我们将已克隆的大肠杆菌碱性磷酸酶基因重组到抗HBsAg Fab段的Fd羧基端,构建了重组融合蛋白表达载体pHBFAP,转化大肠杆菌XLl-Blue,经IPTG诱导表达后,采用ELISA法检测到培养上清中存在与HBsAg的结合活性和碱性磷酸酶的催化活性,显示抗HBsAg-碱性磷酸酶双功能抗体分子在大肠杆菌中获得了表达. To construct a bifunctional engineered antibody with alkaline phosphatase activity, we cloned the E. coli alkaline phosphatase gene to the Fd carboxyl terminal of the anti-HBsAg Fab fragment to construct a recombinant fusion protein expression vector pHBFAP. After induced by IPTG, the activity of HBsAg and alkaline phosphatase in the culture supernatant were detected by ELISA. The results showed that anti-HBsAg-alkaline phosphatase bifunctional antibody was expressed in Escherichia coli In the expression.