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目的探究微量元素硒和病原微生物感染与宫颈癌发病的相关性。方法采集行手术治疗的宫颈癌患者标本共180份。同时选取80份非宫颈癌患者正常宫颈组织作为对照组。采用聚合酶链式反应法(PCR)对宫颈癌组织中解脲支原体、人乳头瘤病毒、EB病毒、疱疹病毒、沙眼衣原体及人巨细胞病毒的感染进行检测。采用酶联免疫法对血清中肿瘤坏死因子和白细胞介素-2R的含量进行检测。采用荧光分光光度法对血清中微量元素硒的含量进行检测。结果 180份宫颈癌患者的癌组织标本中,153份为病原微生物阳性感染,阳性感染率为85.0%。80份非宫颈癌患者正常宫颈组织中,16份为病原微生物阳性感染,阳性感染率为20.0%。宫颈癌患者标本血清中肿瘤坏死因子和白细胞介素-2R水平均高于对照组,硒含量低于对照组(P<0.05)。结论病原微生物感染与宫颈癌的发病密切相关,同时血清中会出现肿瘤坏死因子和白介素-2R水平上升和硒含量降低。
Objective To explore the correlation between trace element selenium and pathogenic microorganism infection and the incidence of cervical cancer. Methods A total of 180 specimens of cervical cancer were collected. At the same time select 80 non-cervical cancer patients with normal cervical tissue as a control group. The infection of Ureaplasma urealyticum, human papillomavirus, Epstein-Barr virus, herpesvirus, Chlamydia trachomatis and human cytomegalovirus in cervical cancer tissues was detected by polymerase chain reaction (PCR). Serum tumor necrosis factor and interleukin-2R levels were detected by enzyme-linked immunosorbent assay. The content of trace element selenium in serum was detected by fluorescence spectrophotometry. Results Of the 180 specimens of cervical cancer, 153 were positive for pathogenic microorganisms and the positive rate was 85.0%. Among 80 non-cervical cancer patients, 16 were positive for pathogenic microorganisms, and the positive rate was 20.0%. Cervical cancer patients serum tumor necrosis factor and interleukin -2R levels were higher than the control group, selenium content was lower than the control group (P <0.05). Conclusion The infection of pathogenic microorganisms is closely related to the pathogenesis of cervical cancer. At the same time, serum tumor necrosis factor and interleukin-2R levels rise and selenium levels decrease.