本文以 TLC、HPLC、UV 及 GC—MS 联用技术测定了甲基睾丸素及一系列标准品中其它甾体杂质的含量。实验部分:对一系列甲基睾丸素样品及世界卫生组织(WHO)、欧洲药典(Europ.P)、英国药典(B、P)、美国国家药品处方集(NF)等所提供的标准品加以分析。用 TLC 法作预试验。采用 Silufol VU—254预制层析板(150×150mm),以已烷—丙酮(70:30)的混合液 The contents of methyl steroids and other steroid impurities in a series of standards were determined using TLC, HPLC, UV, and GC-MS coupled techniques. Experimental part: A series of methyltestosterone samples and standards provided by the World Health Organization (WHO), European Pharmacopoeia (Europ. P), British Pharmacopoeia (B, P), U.S. National Formulary (NF), etc. analysis. Pretest with TLC method. Silufol VU-254 Pre-Purified Chromatography Plate (150×150mm) with a mixture of hexane-acetone (70:30)