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目的:研究大黄素对哮喘小鼠肺部炎症及肺泡灌洗液(BALF)中炎性细胞因子分泌水平的影响,初探其作用机理。方法:采用卵清蛋白(Ovalbumin,OVA)致敏加激发法建立支气管哮喘小鼠模型。20只雌性C57BL/6小鼠随机分为正常组、模型组、大黄素组及阳性药对照组,实验结束后观察各组小鼠整体情况和肺组织病理学改变,收集肺泡灌洗液进行细胞因子检测。结果:模型组小鼠可见呼吸急促、喘息、打喷嚏、抓耳挠鼻、弓背、大小便失禁等症状,BALF中白介素(Interleukin,IL)-4、IL-5、IL-17A含量明显升高(P<0.01);肺组织病理学结果为支气管及血管周围大量炎性细胞浸润,肺泡结构不完整,伴支气管上皮损坏甚至纤维化等明显改变。经大黄素治疗后,哮喘小鼠上述症状、体征及病理改变均有所改善,且炎性细胞因子水平明显降低。结论:大黄素能够在一定程度上改善支气管哮喘小鼠肺部的症状及病理变化,抑制IL-4、IL-5、IL-17A的分泌。
Objective: To study the effects of emodin on lung inflammation and the secretion of inflammatory cytokines in bronchoalveolar lavage fluid (BALF) in asthmatic mice and to explore its mechanism. Methods: Bronchial asthma model was induced by ovalbumin (OVA) sensitization and stimulation. Twenty female C57BL / 6 mice were randomly divided into normal group, model group, emodin group and positive drug control group. After the experiment, the overall condition and pathological changes of the mice in each group were observed. The bronchoalveolar lavage fluid Factor detection. Results: The levels of interleukin (IL) -4, IL-5 and IL-17A in BALF were significantly increased in the model group as compared with those in the untreated group, with the symptoms of shortness of breath, wheezing, sneezing, scratching the ear and nose, (P <0.01). The results of lung histopathology showed that a large number of inflammatory cells infiltrated the bronchi and perivascular, the alveolar structure was incomplete, the bronchial epithelium damaged or even the fibrosis was obviously changed. After emodin treatment, the above symptoms, signs and pathological changes in asthmatic mice were improved, and the levels of inflammatory cytokines were significantly reduced. Conclusion: Emodin can ameliorate the lung symptoms and pathological changes of bronchial asthma mice and inhibit the secretion of IL-4, IL-5 and IL-17A.