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目的研究HIV/AIDS患者血液中的IL-17/23在外周血中的表达情况、IL-17/23含量检测在治疗HIV/AIDS患者中的应用价值。方法采用双抗体夹心酶联免疫吸附(ABC-ELISA)法检测健康人群与艾滋病患者血清IL-17和IL-23水平,同时检测外周血淋巴细胞CD4计数,分析IL-17/23含量与CD4细胞计数之间的相关性。结果 HIV感染者外周血中IL-17、IL-23的浓度及CD4细胞计数水平分别是(39.31±8.26)pg/ml、(8.25±3.11)pg/ml、(462±78)个/μl,AIDS患者外周血中IL-17、IL-23的浓度及CD4细胞计数水平分别是(25.75±11.75)pg/ml、(7.21±2.68)pg/ml、(125±81)个/μl,与正常组比较IL-17、IL-23含量及CD4细胞计数在HIV感染者、AIDS患者外周血中的表达水平均显著降低,差异具有统计学意义(P<0.05)。结论 IL-17/23在HIV/AIDS患者外周血中低表达,IL-17/23含量与CD4细胞计数存在正相关性,通过检测IL-17/23含量能够间接反应HIV/AIDS患者机体免疫状态。
Objective To study the expression of IL-17/23 in peripheral blood of HIV / AIDS patients and the value of IL-17/23 assay in the treatment of HIV / AIDS. Methods Serum levels of IL-17 and IL-23 in healthy and AIDS patients were measured by double antibody sandwich enzyme-linked immunosorbent assay (ABC-ELISA). CD4 counts of peripheral blood lymphocytes were also measured. The levels of IL-17/23 and CD4 Correlation between counts. Results The levels of IL-17, IL-23 and CD4 in peripheral blood of HIV-infected patients were (39.31 ± 8.26) pg / ml, (8.25 ± 3.11) pg / ml and (462 ± 78) The levels of IL-17, IL-23 and CD4 in peripheral blood of AIDS patients were 25.75 ± 11.75 pg / ml, 7.21 ± 2.68 pg / ml and 125 ± 81 / The levels of IL-17, IL-23 and CD4 counts in peripheral blood of HIV-infected and AIDS-infected patients were significantly lower than those of controls (P <0.05). Conclusion IL-17/23 is lowly expressed in peripheral blood of HIV / AIDS patients, and there is a positive correlation between IL-17/23 and CD4 cell count. The IL-17/23 level can indirectly reflect the immune status of HIV / AIDS patients .