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目的:通过季节性流感监测,对流感病毒进行抗原性和基因特性分析,及时发现变异病毒和新型毒株,预测流感流行趋势。方法:采用PCR方法进行流感病毒检测,采用MDCK细胞培养法分离流感病毒。结果:从锦州市2007年~2009年季节性流感监测结果来看,2007年和2008年流感毒株分离率较低,分别为6%和8.2%,而2009年流感毒株分离率相对较高,为11.36%。结论:锦州市2007年和2008年的流感流行主要在秋冬季节,主要以甲型H3和BY型为主;2009年全年均有流感毒株出现,主要以甲型为主,并在季节性流感监测中检测出引起世界大流行的新型毒株甲型H1N1型。
OBJECTIVE: To analyze the antigenicity and genetic characteristics of influenza virus through seasonal influenza surveillance, and discover the mutant virus and new strain in time to predict the epidemic trend of influenza. Methods: The influenza virus was detected by PCR and the influenza virus was isolated by MDCK cell culture. Results: From 2007 to 2009, the results of seasonal influenza surveillance in Jinzhou showed that the isolation rates of influenza strains in 2007 and 2008 were lower at 6% and 8.2%, respectively, while the isolation rates of influenza strains in 2009 were relatively high , 11.36%. Conclusion: The pandemic influenza epidemic in 2007 and 2008 in Jinzhou was mainly in the autumn and winter seasons, mainly Influenza A and B types. In 2009, influenza strains appeared mainly in type A, Influenza A (H1N1), a new type of strain causing the global pandemic, was detected during influenza surveillance.