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本研究以~3H哌唑嗪为特异的标记配基定量地检测了家兔心脏α_1受体及其在心脏缺血/再灌注损伤时的变化,并结合细胞膜内Ca~(2+)含量的变化及丹参对缺血心肌的保护作用,探讨了心肌/再灌注损伤的机理。结果表明,α_1受体随心肌缺血时间的延长而明显升高,40分钟时达正常对照的2倍以上,在此基础上再灌注4分钟,受体数目稍降,但仍保持在高水平。细胞膜的Ca~(2+)在缺血时的变化与α_1受体的变化平行(随时间而增高),但缺血40分钟再灌注4分钟时明显下降。预先耳缘静脉注射丹参制剂后,缺血40分钟再灌注4分钟,上述两种指标均与对照组无差异。提示丹参对心肌细胞膜具有明显保护作用。
In this study, 3H-prazosin-specific labeled ligand was used to quantitatively detect the changes of α_1 receptor in the heart of rabbits and its effect on myocardial ischemia / reperfusion injury. Combined with the intracellular Ca 2+ content Changes and Salvia on the protective effect of ischemic myocardium to explore the myocardial / reperfusion injury mechanism. The results showed that the α_1 receptor was significantly increased with prolongation of myocardial ischemia, reaching more than 2 times of that of the normal control at 40 minutes. On the basis of 4 minutes after reperfusion, the number of receptors decreased slightly but remained at a high level . Changes of Ca2 + in the plasma membrane were parallel to the changes of the α1 receptors (increased with time), but significantly decreased at 4 minutes after reperfusion at 40 minutes of ischemia. Danshen injection of the ear before the ear vein preparation, ischemia reperfusion for 40 minutes 4 minutes, the above two indicators were no difference with the control group. Salvia miltiorrhiza on myocardial cell membrane has obvious protective effect.