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用新鲜人血液经肝素抗凝,于4℃下制备红细胞膜,悬浮于5mmol磷酸缓冲液(pH7.5)和50%甘油混合液中,蛋白浓度约4—5mg/ml,贮存于-20℃备用。3H-cAMP(中国原子能研究院产品,14Ci/mmol,
Fresh human blood was anticoagulated with heparin and erythrocyte membranes were prepared at 4 ° C and suspended in a mixture of 5 mM phosphate buffer (pH 7.5) and 50% glycerol at a protein concentration of about 4-5 mg / ml and stored at -20 ° C spare. 3H-cAMP (China Atomic Energy Research Institute, 14Ci / mmol,