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本研究建立了在同一PCR反应条件下同时检测人血小板抗原 (humanplateletantigen ,HPA)系统HPA 1到HPA 5的PCR SSP检测法。应用该方法分析了 110例健康献血员的HPA 1到HPA 5的基因型 ,并以此为依据推算了中国人HPA 1到HPA 5各亚型的基因频率。结果表明HPA 1a和HPA 1b的基因频率分别为 0 .91和 0 .0 9,HPA 2a和HPA 2的基因频率分别为 0 .86和 0 .14,HPA 3a和HPA 3b的基因频率分别为 0 .60和 0 .4 0 ,HPA 4a和HPA 4b的基因频率分别为 0 .92和 0 .0 8,HPA 5a和HPA 5b的基因频率分别为 0 .85和 0 .15。结论 :基因组DNA的血小板抗原PCR SSP分型法切实可行 ,可广泛应用于临床血小板抗原的分型
In this study, we established a PCR SSP method for the simultaneous detection of HPA 1 to HPA 5 from human platelet antigen (HPA) system under the same PCR reaction conditions. Using this method, the genotypes of HPA 1 to HPA 5 in 110 healthy blood donors were analyzed and the frequency of Chinese HPA 1 to HPA 5 subtypes was calculated. The results showed that the gene frequencies of HPA 1a and HPA 1b were 0.91 and 0. 9, respectively. The frequencies of HPA 2a and HPA 2 genes were 0.86 and 0.14, respectively. The frequencies of HPA 3a and HPA 3b genes were 0 .60 and 0.40, respectively. The gene frequencies of HPA 4a and HPA 4b were 0.92 and 0.08, respectively. The gene frequencies of HPA 5a and HPA 5b were 0.85 and 0.15, respectively. Conclusion: Genomic DNA platelet antigen PCR SSP typing method is feasible and can be widely used in the clinical classification of platelet antigens