论文部分内容阅读
目的研究内皮抑素和多西环素对黑色素瘤生长及肿瘤细胞基质金属蛋白酶-9(MMP-9)、-2(MMP-2)及基质金属蛋白酶组织抑制因子(TIMP-2)表达水平的影响。方法C57/BL6小鼠57只,建立小鼠B16黑色素瘤动物模型,分多西环素组、多西环素加内皮抑素组,内皮抑素组和对照组4组,给予内皮抑素和多西环素处理,比较肿瘤的体积大小及生长速度,免疫组织化学染色检测肿瘤组织MMP-9、MMP-2及TIMP-2的表达。结果多西环素组、多西环素加内皮抑素组和内皮抑素组肿瘤均较对照组生长缓慢(F=4.32,P<0.05),其中多西环素组、多西环素加内皮抑素组和对照组之间肿瘤平均生长体积差异有统计学意义(t=2.40,t=2.58;P<0.05)。MMP-2、MMP-9和TIMP-2在各处理组的表达与在对照组的表达之间差异均有统计学意义(F=12.79,F=5.56,F=4.64;P<0.05)。结论多西环素和内皮抑素联合使用,影响肿瘤组织MMPs及其抑制剂的表达,明显抑制黑色素瘤生长和局部浸润转移。
Objective To investigate the effects of endostatin and doxycycline on the growth of melanoma and the expression of matrix metalloproteinase-9 (MMP-2), TIMP-2 and tumor necrosis factor-2 influences. Methods Fifty-seven C57 / BL6 mice were divided into 3 groups: Doxocetin group, Doxycycline plus Endostatin group, Endostatin group and control group. Animal models of B16 melanoma were established. Endostatin and Doxycycline treatment, tumor size and growth rate were compared. The expression of MMP-9, MMP-2 and TIMP-2 in tumor tissues was detected by immunohistochemical staining. Results Compared with the control group, the tumors in the doxycycline group, doxycycline plus endostatin group and endostatin group grew slowly (F = 4.32, P <0.05), among which, the doxycycline group, doxycycline plus The mean growth volume of tumor between endostatin group and control group was statistically significant (t = 2.40, t = 2.58; P <0.05). There was significant difference between the expression of MMP-2, MMP-9 and TIMP-2 in all treatment groups and the control group (F = 12.79, F = 5.56, F = 4.64, P <0.05). Conclusion The combination of doxycycline and endostatin can affect the expression of MMPs and its inhibitors in tumor tissue and significantly inhibit the growth of melanoma and the local invasion and metastasis.