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目的:建立佛耳草的质量控制方法,为江苏省中药材标准佛耳草质量修订提供依据。方法:采用性状、显微鉴别、薄层色谱鉴别、水分测定、浸出物测定及含量测定对佛耳草进行质量评价。采用HPLC法测定佛耳草中槲皮素和木犀草素的含量。色谱柱为Diamonsil C18柱(4.6 mm×200 mm,5μm);流动相:甲醇-0.4%磷酸溶液(42∶58);检测波长为360 nm。结果:显微鉴别特征性强;薄层色谱斑点清晰,分离度好。含量测定中槲皮素在0.041 03~0.820 6μg、木犀草素在0.041 76~0.835 2μg范围内与峰面积呈良好的线性关系;平均加样回收率分别为98.3%、98.9%,RSD分别为1.2%、1.5%。结论:该法简便,准确、重复性好,各成分分离良好,可用于控制佛耳草的质量。
OBJECTIVE: To establish the quality control method of Codonopsis, which provides the basis for the quality revision of Codonin, a standard of Chinese herbal medicine in Jiangsu Province. Methods: The quality of Codonopsis ostreatus was evaluated by traits, microscopic identification, TLC identification, water determination, extract determination and content determination. Determination of Quercetin and Luteolin in Beringia by HPLC. The column was Diamonsil C18 column (4.6 mm × 200 mm, 5 μm). The mobile phase was methanol-0.4% phosphoric acid solution (42:58). The detection wavelength was 360 nm. Results: Microscopic identification of strong; thin layer chromatography spots clear, good resolution. The content of quercetin in the determination of quercetin was 0.041 03 ~ 0.820 6μg, luteolin in the range of 0.041 76 ~ 0.835 2μg and the peak area was a good linear relationship; average recovery was 98.3%, 98.9%, RSD 1.2 %, 1.5%. Conclusion: The method is simple, accurate and reproducible, and the components are well separated and can be used to control the quality of