论文部分内容阅读
许多外科医生在剖腹探查时用晶体液冲洗腹腔以稀释残存的细菌和促使细菌生长的物质(如坏死组织,钡剂,胆汁和血红蛋白)。本文作者制作大肠杆菌腹膜炎的大白鼠模型,观察腹腔内注入盐水对细菌生长、白细胞吞噬和腹腔清除的影响。取体重200g～250g的雄性大鼠,腹腔内分别注入1、10、20或30ml内含5×10~8个活大肠杆菌的生理盐水。48小时后计算死亡率。对照组腹腔内注入含等量活大肠杆菌的生理盐水1ml,同时皮下注入30ml无菌盐水,间隔一定时间先后处死。剖腹后测定腹腔液容积,然后稀释作活菌落计数。
Many surgeons rinse the peritoneal cavity with crystalloid during laparotomy to dilute residual bacteria and substances that promote bacterial growth (such as necrotic tissue, barium, bile and hemoglobin). The author of this article produced E. coli peritonitis rat model to observe the intraperitoneal injection of saline on bacterial growth, leukocyte phagocytosis and abdominal clearance. Male rats weighing 200 g to 250 g were intraperitoneally injected with 1, 10, 20 or 30 ml of physiological saline containing 5 x 10-8 live E. coli, respectively. Forty-eight hours later mortality was calculated. The control group was injected intraperitoneally with 1 ml of physiological saline containing the same amount of live E. coli, and 30 ml of sterile saline was administered subcutaneously at the same time and sacrificed at intervals. Abdominal fluid volume was measured after caesarean section, and then diluted to make live colony count.