[目的]为研制高效生物降解菌剂奠定基础。[方法]在60℃条件下对猪粪中纤维素分解菌进行了分离研究,对分离到的菌株进行羧甲基纤维素酶活(CMCase)和滤纸酶活(FPase)测定,并进行16SrRNA基因分析。[结果]BC1和BC3号菌株CMCase、Fpase都较高,但相差不大,通过16SrDNA序列分析发现BC1和BC3号菌株分别与Pseudomonas sp.的相似性达到98%和99%。[结论]该研究为研制高效生物增温剂奠定了基础。 [Objective] To lay the foundation for the development of efficient biodegradable bacteria agents. [Method] The cellulolytic bacteria in swine manure were separated at 60 ℃, the CMCase and FPase activity of the isolated strains were determined, and the 16S rRNA gene analysis. [Result] The CMCase and Fpase of BC1 and BC3 strains were higher but not much different. By 16S rDNA sequence analysis, the similarity of BC1 and BC3 strains to Pseudomonas sp. Was 98% and 99% respectively. [Conclusion] This study laid the foundation for the development of bio-warming agent.