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目的探讨宫颈癌人乳头瘤病毒(HPV)感染与T细胞亚群、CD4+CD25+Foxp3+调节性T细胞(Treg)的相关性。方法采用HPV核酸扩增分型检测试剂盒及流式细胞术检测宫颈癌组(40例)、正常对照组(20例)的HPV分型和外周血中T细胞亚群及CD4+CD25+Foxp3+Treg的百分率。结果 HPV阳性的患者外周血T细胞亚群与HPV阴性的患者比较,差异无统计学意义(P>0.05),但调节性T细胞(Treg)在HPV阳性与阴性组之间比较,差异有统计学意义(P<0.05)。宫颈癌组外周血中CD4+CD25+Foxp3+Treg的百分率与正常对照组比较,差异有统计学意义(P<0.05)。HPV与CD4+CD25+Foxp3+Treg细胞呈显著正相关(r=0.613,P<0.05)。结论宫颈癌患者免疫功能与正常人无明显差异,Treg细胞与宫颈癌患者HPV持续感染、肿瘤免疫逃避密切相关。
Objective To investigate the relationship between HPV infection and T cell subsets and CD4 + CD25 + Foxp3 + regulatory T cells (Tregs) in cervical cancer. Methods HPV genotyping kit and flow cytometry were used to detect HPV types and T cell subsets and CD4 + CD25 + Foxp3 in 40 cases of cervical cancer group and 20 cases of normal control group + Treg percentage. Results There was no significant difference in T cell subsets between peripheral blood and HPV-negative patients in HPV-positive patients (P> 0.05). However, the difference of Tregs between HPV positive and negative groups was statistically significant Significance (P <0.05). The percentage of CD4 + CD25 + Foxp3 + Treg in peripheral blood in cervical cancer group was significantly higher than that in normal control group (P <0.05). HPV was positively correlated with CD4 + CD25 + Foxp3 + Treg cells (r = 0.613, P <0.05). Conclusion There is no significant difference in immune function between cervical cancer patients and normal controls. Treg cells are closely associated with persistent HPV infection and tumor immune evasion in patients with cervical cancer.