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目的采用HPLC-ELSD法测定浙贝母花中贝母素甲和贝母素乙的含量。方法色谱柱为Zorbax Eclipse XDB-C18(150 mm×4.6 mm,5μm),流动相为乙腈-0.1%二乙胺溶液,梯度洗脱。结果贝母素甲、贝母素乙的线性范围分别为0.25~2.0、0.10~0.80μg·m L-1;加样回收率分别为96.11%、91.15%,RSD分别为4.51%、5.79%(n=6);测定了14批浙贝母花中贝母素甲、贝母素乙的含量。结论所用方法准确、简便、灵敏度高,适用于浙贝母花药材的含量测定和质量评价。
OBJECTIVE To determine the contents of peimine and peiminine B in Fritillaria cirrhosa by HPLC-ELSD. Methods The column was Zorbax Eclipse XDB-C18 (150 mm × 4.6 mm, 5 μm). The mobile phase consisted of acetonitrile-0.1% diethylamine solution with gradient elution. Results The linear ranges of Fritillariae and Peiminine B were 0.25-2.0 and 0.10-0.80μg · m L-1, respectively. The recovery rates were 96.11% and 91.15%, respectively. The RSDs were 4.51% and 5.79% n = 6); 14 batches of Fritillaria Fritillaria in a determination of the mother of pearl B content. Conclusion The method is accurate, simple and sensitive, suitable for the determination and quality evaluation of Fritillaria.