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目的研究茉莉酸甲酯(methyl jasmonate,MJ)对丹参毛状根生长和其中丹参素、迷迭香酸、丹酚酸B3种化合物含量的影响。方法以MJ浓度和添加时间为2个影响因素,设计5个水平的正交实验,观察不同MJ浓度和添加时间对丹参毛状根生长和丹参素、迷迭香酸、丹酚酸B3种化合物含量的影响。结果 MJ的加入对丹参毛状根生长有一定抑制作用,并能显著改变丹参素、迷迭香酸、丹酚酸B的含量。在接种第15天添加浓度为300μmol·L-1MJ、共培养15d的毛状根中丹参素含量达(0.510±0.054)%,约为对照样品(0.179±0.02)%的2.85倍;接种第20天加入浓度为300μmol·L-1MJ、共培养10d的毛状根中迷迭香酸的含量达(14.358±0.710)%,约为对照样品(7.021±0.403)%的2倍;接种0天时添加200μmol·L-1MJ、共培养30d的丹参毛状根中丹酚酸B的含量高达(1.592±0.120)%,约为对照样品(0.189±0.05)%的8.42倍。结论茉莉酸甲酯能明显改变丹参素、迷迭香酸、丹酚酸B在丹参毛状根中的积累,并且随MJ浓度和添加时间的改变呈现一定规律性,这对丹酚酸类化合物在植物体内生物合成途经的研究有一定借鉴意义。
Aim To investigate the effects of methyl jasmonate (MJ) on the growth of Salvia miltiorrhiza hairy root and the contents of three compounds, Danshensu, Rosmarinic acid and Salvianolic acid. Methods MJ concentration and adding time were two influencing factors. Five levels of orthogonal experiment were designed. The effect of different MJ concentration and adding time on the growth of Salvia miltiorrhiza hairy root and the effects of Danshensu, rosmarinic acid, salvianolic acid B3 compounds Effect of content. Results The addition of MJ could inhibit the growth of hairy root of Salvia miltiorrhiza and could significantly change the content of Danshensu, rosmarinic acid and salvianolic acid B. At the 15th day of inoculation, the concentration of Danshensu in the hairy roots at the concentration of 300μmol·L-1MJ was (0.510 ± 0.054)%, which was about 2.85 times that of the control (0.179 ± 0.02)%, The content of rosmarinic acid in the hairy roots at the concentration of 300μmol·L-1MJ for 10 days was (14.358 ± 0.710)%, which was about twice as that of the control (7.021 ± 0.403)%, and at 0 days after inoculation The content of salvianolic acid B in hairy roots of Salvia miltiorrhiza Bge cultivated for 30 days was up to (1.592 ± 0.120)%, which was about 8.42 times of the control (0.189 ± 0.05)%. Conclusion Methyl jasmonate can obviously change the accumulation of Danshensu, rosmarinic acid and salvianolic acid B in the hairy roots of Salvia miltiorrhiza, and show certain regularity with the change of MJ concentration and time. In plant biosynthesis pathway has some reference significance.