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氨基酸残基对探针分子的荧光猝灭行为可以为生物大分子的结构及构象动力学研究提供重要的信息.本文运用飞秒瞬态吸收光谱和时间相关单光子计数实验系统研究了在水(H2O)和氘代水(D2O)溶液中乙酰基取代酪氨酸(AcTyr)对Eosin Y的超快荧光猝灭动力学过程.发现导致AcTyr对Eosin Y荧光猝灭的主要原因是由于它们之间形成了短寿命的基态复合物.我们还发现Eosin Y与AcTyr形成的基态复合物的激发态寿命具有明显的动力学同位素效应,表明AcTyr对Eosin Y的荧光猝灭是通过质子耦合电子转移过程发生的.
The fluorescence quenching of probe molecules by amino acid residues can provide important information for the structure and conformational kinetics of biological macromolecules.In this paper, we used the femtosecond transient absorption spectroscopy and the time-dependent single-photon counting experimental system to study the fluorescence quenching behavior of probe molecules in water H2O) and deuterated water (D2O) solution to Eosin Y. The main reason for the fluorescence quenching of Eosin Y by AcTyr was found to be that between them Form a short-lived ground state complex.We also found that the excited state lifetime of the ground state complex formed by Eosin Y and AcTyr has obvious kinetic isotope effect, indicating that fluorescence quenching of Eosin Y by AcTyr occurs through proton-coupled electron transfer of.