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为了探讨脉冲电场(PEF)和X射线联合照射对小鼠雄性生殖和造血器官的复合效应,将74只成年雄性昆明种小鼠随机分成4组,即对照组、PEF组、X射线组以及PEF+X射线复合组。将PEF辐照参数设定为电场强度1 k V/m和重复频率10 k Hz。动物每天接受PEF辐照1次,每次辐照时间为10 s,连续辐照3 d。PEF辐照结束24 h后复合组动物接受X射线全身照射,吸收剂量为7.0 Gy,吸收剂量率为1.1 Gy/min。照射完毕,观察动物体质量、外周血常规指标,睾丸、附睾、脾脏和胸腺脏器系数及睾丸形态结构,脾结节计数,动物15 d存活率。研究结果表明:与对照组相比,PEF组动物体质量、外周血常规指标、睾丸等脏器系数变化不显著,X射线组和复合组动物体质量明显降低,白细胞、淋巴细胞、血小板数量明显减少,胸腺和脾脏系数明显降低,睾丸组织结构损伤明显。与X射线组相比,复合组动物体质量、血液常规指标和脾结节数目变化不显著,但睾丸组织病理损伤明显减轻,动物15 d存活率有增高趋势。可得结论:单独脉冲电场对小鼠雄性生殖器官和造血器官无明显影响,并且对X射线引起的造血损伤无复合效应,但对X射线造成的睾丸组织损伤具有一定程度的保护作用。
To investigate the combined effects of pulsed electric field (PEF) and X-ray irradiation on male reproductive and hematopoietic organs in mice, 74 adult male Kunming mice were randomly divided into 4 groups: control group, PEF group, X-ray group and PEF + X-ray combination group. The PEF irradiation parameters are set to an electric field strength of 1 kV / m and a repetition frequency of 10 k Hz. The animals were exposed to PEF once a day, each irradiation time of 10 s, continuous irradiation 3 d. The animals in the composite group received X-ray whole body irradiation 24 hours after the end of PEF irradiation, with an absorbed dose of 7.0 Gy and an absorbed dose rate of 1.1 Gy / min. At the end of irradiation, the animal body weight, peripheral blood routine indexes, testis, epididymis, spleen and thymus organ coefficient and testicular morphological structure, spleen nodule count and animal 15-day survival rate were observed. The results showed that compared with the control group, there was no significant change in body weight, peripheral blood indexes and testis and other organ coefficients in the PEF group. The body weight of the X-ray group and the composite group was significantly decreased, and the number of white blood cells, lymphocytes and platelets was significantly Reduce, thymus and spleen coefficient was significantly reduced, testicular tissue damage significantly. Compared with the X-ray group, the body weight, blood routine index and spleen nodule number in the composite group had no significant changes, but the pathological damage of testis tissue was significantly reduced. The survival rate of the animals increased at 15 days. It can be concluded that the single pulsed electric field has no obvious effect on the male reproductive organs and hematopoietic organs in mice, and has no combination effect on the hematopoietic damage caused by X-ray, but has a certain degree of protective effect on the testicular tissue damage caused by X-ray.