本方法适用于动物组织中Vit D的含量分析,其含量很少乃动物組織的特征,可測出3—4微克Vit D。本方法在于用硷水介組織,用洋地黃皂甙除去固醇,用Al_2O_3吸附柱除去VitA和其它多烯物,最后用紙上色层分离法从VitD中除去干扰性杂质,借助于SbCl_3的反应进行紙上提取的維生素的测定。若样品用血液,則从正常或有佝偻病的动物中各取血20及40ml,或更 This method is applicable to the analysis of Vit D content in animal tissues. The content of Vit D is very small, which is the characteristic of animal tissues. It can measure 3-4 μg Vit D. The method consists of removing the sterols with digitonin, removing VitA and other polyenes using an Al 2 O 3 adsorption column, and finally removing the interfering impurities from the VitD by chromatography on the paper with the aid of SbCl 3 . Determination of vitamins extracted from paper. If the sample uses blood, take 20 and 40 ml of blood from normal or rickets animals, or more