The cGAS-MITA pathway of cytosolic DNA sensing plays essential roles in immune response against pathogens that contain DNA or with DNA production in their life cycles.The cGAS-MITA pathway also detects leaked or aberrant accumulated self DNA in the cytoplasm under certain pathological conditions,such as virus induced cell death,DNA damage,mitochondria damage,gene mutations,which results in autoimmune diseases.Therefore,the cGAS-MITA pathway must be tightly controlled to ensure proper immune response against pathogens and to avoid autoimmunediseases.The regulation of cGAS-MITA pathway at MITA-level have been extensively explored and reviewed elsewhere,here we provide a summary and perspective on recent advances in understanding of the cGAS regulation.