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2008年4月从广州出入境检验检疫局送检的越南进境人参果样品(报检号:P02297)中发现果实表面密布近圆形褐色病斑,病斑直径约为3mm,中央黄褐色,边缘深红褐色,多个病斑可愈合成片。室温下放置1周,病果即完全腐烂,全果果皮及果肉均转成褐色。采用组织分离培养法获得分离物,经对分离物培养性状与形态特征观察、致病性测定,并辅助于分子生物学检测,将病害鉴定为人参果炭疽病,病原菌为胶孢炭疽菌(Colletotrichum gloeosporioides)。B last分析表明从病原菌基因组中扩增到的1.8 kbp 18S rDNA片段与胶孢炭疽菌18S rRNA基因同源率为99%。迄今为止人参果炭疽病在国内外均未见报道,这是我国首次从进境人参果中截获并检验鉴定该病害。
In April 2008, a sample of Vietnamese entry ginseng samples (No.222: 22) submitted by the Guangzhou Exit-Entry Inspection and Quarantine Bureau found that the surface of the fruit was dense and nearly round brown with a lesion diameter of about 3 mm. The central yellowish brown edge Dark reddish brown, multiple lesions can heal into tablets. Placed at room temperature for 1 week, the fruit is completely decayed, the whole fruit peel and pulp are turned brown. The isolates were isolated by tissue culture method. The traits and morphology of the isolates were observed, the pathogenicity was determined, and the molecular biology was used to detect the isolates. The disease was identified as anthrax ginseng and the pathogen was Colletotrichum gloeosporioides ). B last analysis revealed that the 1.8 kbp 18S rDNA fragment amplified from the genome of the pathogen had 99% identity with the 18S rRNA gene from Colletotrichum gloeosporioides. So far, ginseng fruit anthracnose has not been reported at home and abroad, which is the first time that China has intercepted and tested and identified the disease from the entry ginseng fruit.