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对在胃癌高表达的EPH家族基因erk胞外的配体结合区基因扩增及克隆．方法：从胃癌患者手术切除标本中提取RNA，反转录为cDNA，以计算机辅助分析设计并合成引物进行RT－PCR，并将扩增片段克隆入pUC19质粒，用酶切及PCR方法筛选鉴定阳性克隆．结果：RT－PCR扩增片段与设计相符，且特异性好，无非特异产物出现，表明所设计引物符合要求．酶切及巢式PCR、PCR－RFLP方法证明克隆片段正确插入pUC19的HindIII和BamHI位点，并将重组克隆命名为pGE42．结论；克隆的完成为进一步研究该基因在胃癌的发生中的作用，寻找其胞外配体及进行表达产物的细胞定位等工作打下基础．
For the ERK family of gene Erk highly expressed in gastric cancer, the extracellular ligand binding domain gene was amplified and cloned. Methods: RNA was extracted from surgical specimens of patients with gastric cancer and reverse transcribed into cDNA. RT-PCR was designed and synthesized by computer-assisted analysis. The amplified fragments were cloned into pUC19 plasmid and identified by enzyme digestion and PCR. clone. Results: RT-PCR amplified fragments were consistent with the design, and the specificity was good. No non-specific products appeared, indicating that the designed primers met the requirements. Enzymatic digestion and nested PCR, PCR-RFLP method proved that the cloned fragment was correctly inserted into the HindIII and BamHI sites of pUC19, and the recombinant clone was named pGE42. Conclusion: The completion of cloning lays the foundation for the further study of the role of this gene in the development of gastric cancer, searching for its extracellular ligands and performing cellular localization of expression products.