论文部分内容阅读
目的研究雄激素受体(AR)阴性人前列腺癌PC-3细胞系再表达人全长AR cDNA,即PC-3-AR+细胞系生长因子相关基因族的表达情况,探讨雄激素依赖型和非依赖型前列腺癌细胞AR与生长因子信号途径的相关性。方法采用PCR芯片技术(PCR-array)进行研究,利用德国Qiagen公司的RT~2 Profiler~(TM)PCR Array Human Growth Factors板对PC-3、PC-3-AR+两株细胞系的生长因子相关基因族的表达量进行相对定量分析,之后通过数据分析,比较基因表达的差异。结果两株人前列腺癌细胞系PC-3和PC-3-AR+中生长因子相关基因族的表达有明显差异性,其中FGF13、IGF2、CSF2、CXCL1等基因表达差异倍数超过5倍以上,具有显著性差异(P<0.01)。结论人前列腺癌细胞系PC-3再表达雄激素受体AR影响其生长因子相关基因族的表达,人前列腺癌AR与生长因子表达信号通路具有相关性。
Objective To study the expression of human full-length AR cDNA, the PC-3-AR + cell line growth factor-related gene family, in androgen receptor (AR) negative human prostate cancer PC-3 cell lines, Correlation between AR and Growth Factor Signaling Pathway in Dependent Prostate Cancer Cells. Methods PCR-array was used to study the correlation between the growth factors of PC-3 and PC-3-AR + cell lines by RT ~ 2 Profiler TM PCR Array Human Growth Factors (Qiagen) Relative quantitative analysis of gene expression levels, then through data analysis, comparison of gene expression differences. Results The expression of growth factor-related genes in PC-3 and PC-3-AR + cells was significantly different between the two human prostate cancer cell lines. The difference in gene expression of FGF13, IGF2, CSF2 and CXCL1 was more than 5 times, which was significant Sex differences (P <0.01). Conclusions The human prostate cancer cell line PC-3 re-expresses androgen receptor AR affects the expression of its growth factor-related gene family, and the correlation between AR and the expression of growth factor in human prostate cancer cells.