AIM Our previous research on the surgical samples ofprimary liver cancer with CGH showed that the loss of humanchromosome 8p had correlation with the metastaticphenotype of liver cancer.In order to seek the functionalevidence that there could be a metastatsis suppressor gene(s)for liver cancer on human chromosome 8,we tried totransfer normal human chromosome 8 into rat liver cancercell line C5F,which had high metastatic potential to lung.METHODS:Human chromosome 8 randomly marked withneo gene was introduced into C5F cell line by MMCT andpositive microcell hybrids were screened by double selectionsof G418 and HAT.Single cell isolation cloning was appliedto clone microcell hybrids.Finally,STS-PCR and WCP-FISHwere used to confirm the introduction.RESULTS:Microcell hybrids resistant to HAT and G418 wereobtained and 15 clones were obtained by single-cell isolationcloning.STS-PCR and WCP-FISH proved that humanchromosome 8 had been successfully introduced into ratliver cancer cell line C5F.STS-PCR detected a random lossin the chromosome introduced and WCP-FISH found aconsistent recombination of the introduced humanchromosome with the rat chromosome.CONCLUSION:The successful introduction of humanchromosome 8 into highly metastatic rat liver cancer cellline builds the basis for seeking functional evidence of ametastasis suppressor gene for liver cancer harboring onhuman chromosome 8 and its subsequent cloning. AIM Our previous research on the surgical samples of primary liver cancer with CGH showed that the loss of human chromosome 8p had correlation with the metastatic phenotype of liver cancer. In order to seek the functionalevidence that there could be a metastatsis suppressor gene (s) for liver cancer on human chromosome 8, we tried totransfer normal human chromosome 8 into rat liver cancer cell line C5F, which had high metastatic potential to lung. METHODS: Human chromosome 8 randomly marked with neo gene was introduced into C5F cell line by MMCT and positive for microcell hybrids were screened by double Selections of G418 and HAT.Single cell isolation cloning was applied to clone microcell hybrids. Finaally, STS-PCR and WCP-FISHwere used to confirm the introduction .RESULTS: Microcell hybrids resistant to HAT and G418 wereobtained and 15 clones were obtained by single-cell isolationcloning .STS-PCR and WCP-FISH proved that human chromosome 8 had been successfully introduced into ratliver cancer cell line C5F.ST S-PCR detected a random lossin the chromosome introduced and WCP-FISH found aconsistent recombination of the introduced human chromosome with the rat chromosome. CONCLUSION: The successful introduction of human chromosome 8 into highly metastatic rat liver cancer cell line builds the basis for seeking functional evidence of ametastasis suppressor gene for liver cancer harboring onhuman chromosome 8 and its subsequent cloning.