Background: Otoscopy examination can be challenging. Traditional teaching uses still image illustrations. Newer attempts use video samples to simulate the otoscopy exam which is a dynamic process.Aims/Objective:To assess whether recorded otoscopy videos from a smartphone adaptable otoscope can be used to develop a video-based otoscopy quiz which may be used for instructing and familiarizing participants to normal anatomy and pathologic ear conditions. To use this quiz to assess current pediatric residents' competency of common otoscopy diagnosis. Method and materials: This study was conducted in 2018. Video samples of ear pathology were collected at the Albany Medical Center using a smartphone adaptable otoscope-Cellscope. The videos were used to create a video otoscopy quiz (VOQ) without clinical vignettes. 45 pediatric residents from 3 academic institutions were evaluated with the quiz. Results: The weighted mean for the VOQ was 66.90％ (95％CI 58.89％-68.42％). The breakdown by questions are: myringosclerosis 72.88％, retraction pocket 80.65％, cholesteatoma 42.22％, hemotympa-num 75.04％, tympanic membrane perforation 79.62％, cerumen impaction 95.46％, otitis externa 52.54％, otitis media with effusion 63.30％, acute otitis media 75.55％, normal ear 36.39％. Conclusion: We found that videos of otoscopy exams can be obtained with a smartphone adaptable otoscope and validated to develop a video-based quiz, which may be used to supplement otoscopic instruction. Following our testing process, we found pediatric residents are relatively well equipped to identify ear pathology on VOQ.