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目的:研究谷胱甘肽复方注射液(CGII)治疗猪血清致大鼠免疫性肝纤维化的药效作用。方法:建立猪血清诱导免疫性肝纤维化模型,全自动生化仪检测血清中丙氨酸氨基转移酶(ALT)、天冬氨酸氨基转移酶(AST)、白蛋白(A)及球蛋白(G)水平;酶免法检测血清中透明质酸(HA)的含量;称取肝脏湿重,计算肝脏指数;消化法检测肝组织羟脯氨酸(Hyp)含量;苏木素-伊红(HE)及Masson染色处理肝脏组织切片;光镜观察病理学改变。结果:在猪血清诱导的大鼠肝纤维化模型中,所有剂量水平的CGII(2.7,5.4,10.8 mg.kg-1,im)能显著降低大鼠血清ALT,AST,A/G值和HA水平;中、高剂量CGII(5.4,10.8 mg.kg-1,im)能显著降低大鼠肝组织Hyp含量,明显改善肝脏病理组织状况;高剂量CGII(10.8 mg.kg-1,im)能显著降低大鼠肝脏指数。结论:CGII对猪血清致大鼠肝纤维化有治疗作用。
Objective: To study the pharmacodynamic effects of glutathione compound injection (CGII) on immune-induced hepatic fibrosis induced by pig serum. Methods: The model of autoimmune liver fibrosis induced by porcine serum was established. Serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), albumin (A) and globulin (HE) in serum were measured by enzyme immunoassay. The wet weight of liver was weighed and the liver index was calculated. The content of hydroxyproline (Hyp) in liver tissue was detected by digestion method. The content of hematoxylin- And Masson staining of liver tissue sections; pathological changes observed by light microscopy. Results: All doses of CGII (2.7, 5.4, 10.8 mg.kg-1, im) significantly reduced the levels of serum ALT, AST, A / G and HA in the rat model of liver fibrosis induced by swine serum (6.4,10.8 mg.kg-1, im) can significantly reduce the Hyp content in rat liver tissue and significantly improve the pathological conditions of liver. High-dose CGII (10.8 mg.kg-1, im) Significantly reduce rat liver index. Conclusion: CGII can treat rat liver fibrosis induced by pig serum.