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采用原子荧光法测定人血、尿、膳食及粪便中硒含量,并比较4个不同膳食类型的城市人体摄取硒的差异。分别在天津、成都、镇江、太原采集30名健康成年男性的全血、72hr尿、72hr膳食和72 hr粪便,在还原剂KBH4浓度为0.37 mol/L,HCl体积分数为5%的条件下采用原子荧光法测定硒含量,方法回收率为97.0%～105.0%,RSD为0.2%～1.2%。结果表明:四个地区均属于硒适宜地区,虽然成都和太原地区的膳食硒含量偏低,但其膳食中硒的摄入量能够满足当地人的需要量。
The content of selenium in human blood, urine, diet and feces was determined by atomic fluorescence spectrometry. The differences of selenium uptake in urban human body from 4 different dietary types were compared. Thirty healthy adult male blood, 72 hr urine, 72 hr diet and 72 hr faeces were collected in Tianjin, Chengdu, Zhenjiang, and Taiyuan, respectively, using KBH4 at a concentration of 0.37 mol / L and a HCl concentration of 5% The determination of selenium content by atomic fluorescence method, the recovery rate was 97.0% ~ 105.0%, RSD was 0.2% ~ 1.2%. The results showed that all four regions belonged to the suitable selenium area. Although the content of dietary selenium in Chengdu and Taiyuan was low, the dietary intake of selenium could meet the needs of locals.