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目的 研究含黄素单氧化酶 3(flavin- containing monooxygenase3,FMO3)基因突变频率在云南少数民族群体中的分布。方法 采用聚合酶链反应 -限制性片段长度多态性方法对云南 2 4个少数民族的 2 8个群体进行了 FMO3基因型分析。结果 FMO3/ Stop1 48频率分布范围为 0 .174～ 0 .80 3,平均为0 .395 ;FMO3/ L ys1 58频率分布范围为 0 .0 5 6～ 0 .4 14 ,平均为 0 .2 0 8;FMO3/ Gly30 8频率分布范围为 0～0 .2 17,其中布朗族、新平花腰傣、德宏水傣、壮族、德昂族、景颇族、怒族和回族的频率为零 ,平均为 0 .0 4 6。结论 FMO3基因突变频率不仅民族间有较大的差异 ,而且同一民族不同群体也不相同。
Objective To investigate the frequency of FMO3 gene mutation in Yunnan ethnic minority population. Methods FMO3 genotypes were analyzed by polymerase chain reaction - restriction fragment length polymorphism (PCR - RFLP) in 28 populations from 24 ethnic minorities in Yunnan Province. Results The frequency distribution of FMO3 / Stop1 48 ranged from 0.174 to 0.80 3 with an average of 0.395. The frequency distribution of FMO3 / L ys1 58 ranged from 0.056 to 0.144 with an average of 0.20 8; FMO3 / Gly30 8 frequency distribution range of 0 ~ 0.22, of which the Blang, Xinhua Huayao Dai, Dehong Shui Dai, Zhuang, Deang, Jingpo, Nu and Hui frequency is zero, with an average of 0 .0 4 6. Conclusion The mutation frequency of FMO3 gene not only has great difference among different ethnic groups, but also different groups of the same ethnic group.