论文部分内容阅读
透射电子显微镜病理诊断技术已广泛用于临床病理诊断中。作者根据国内的实际情况，以充分满足临床病理诊断的要求为中心，针对不同的标本建立了一套实用的电镜病理诊断样品制备标准化技术。该技术包括各种试剂的制备与贮存，取样的要求，５小时包埋程序，２４小时包埋程序，液体标本（穿刺细胞活检标本）包埋程序以及利用石蜡包埋块、石蜡切片制作电镜标本程序等。电镜观察结果显示，经上述方法制备的电镜样品，其组织和细胞的超微结构清晰，完全可以满足临床病理诊断需要。文内还对该技术中具有实用价值的关键步骤进行了讨论
Transmission electron microscopy pathological diagnosis has been widely used in clinical and pathological diagnosis. According to the actual situation in our country, with a focus on meeting the requirements of clinicopathological diagnosis, we have established a set of practical standardized techniques for the preparation of electron microscopic pathological diagnosis samples for different specimens. The technique includes preparation and storage of various reagents, sampling requirements, 5-hour embedding procedure, 24-hour embedding procedure, liquid specimen (aspiration biopsy specimen) embedding procedure, and electron microscopy using paraffin embedded blocks and paraffin sections Program and so on. Electron microscopy results show that the electron microscopy prepared by the above method, the tissue and cell ultrastructure clear, fully meet the needs of clinical and pathological diagnosis. The article also discusses key practical steps in this technology