目的旨在寻找新型抗肿瘤药物，进一步研究ｄ宁烯、丹参及姜黄素衍生物的抗肿瘤机理。采用分子生物学方法及划痕标记染料示踪技术，研究了４种人实体瘤起源的细胞系的细胞间隙信息传导（ＧＪＩＣ）、Ｈｒａｓ癌基因表达以及ｒａｓ癌基因产物（Ｐ２１ｒａｓ蛋白）表达状态，并观察了４种天然产物对它们的影响。结果表明，细胞内染料传输功能的丧失与ｒａｓ基因突变率呈正相关；单萜化合物ｄ宁烯和酚类化合物丹参衍生物的抗肿瘤作用可能与抑制Ｐ２１ｒａｓ蛋白膜结合和增强细胞间隙信息传导功能有关。提示Ｒａｓ癌基因产物Ｐ２１ｒａｓ蛋白膜结合的抑制与细胞间隙信息传导功能的增强有直接关系 The purpose is to find new antitumor drugs and further study the anti-tumor mechanism of d-ningne, salvia miltiorrhiza and curcumin derivatives. Using molecular biology methods and scratch-marking dye tracer techniques, cell gap information transmission (GJIC), Hras oncogene expression, and ras oncogene product (P21ras protein) expression in four cell lines derived from human solid tumors were studied. Status and observed the effects of the four natural products on them. The results showed that the loss of intracellular dye transport function was positively correlated with the mutation rate of ras gene; the antitumor effect of the monoterpene compound d-ningne and phenolic compound salvia miltiorrhizae derivatives may inhibit the binding of P21ras protein membrane and enhance the cell gap information conduction function. related. It is suggested that the inhibition of the membrane binding of the Ras oncogene product P21ras protein is directly related to the enhancement of the cell gap information conduction function