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采用二氧化碳法和强酸法模拟海水酸化效应,研究两种海水酸化模式对中间球海胆早期发育及存活的影响。试验结果显示,两种海水酸化模式均能降低中间球海胆胚胎的上浮率和四腕浮游幼体的存活率;显微观察得知,两种海水酸化模式下,中间球海胆四腕浮游幼体均出现对称性缺失和骨针外露现象,且对称性缺失程度随海水pH的降低而升高;扫描电镜观察发现,两种海水酸化模式下,中间球海胆四腕浮游幼体骨针的表面及横截面均有溶蚀痕迹,且溶蚀程度随海水pH的降低而加剧。结果提示,海水酸化可通过降低胚胎上浮率、浮游幼体存活率以及破坏幼体对称性和骨针结构影响中间球海胆的早期发育及存活。
Carbonate method and strong acid method were used to simulate the acidification effect of seawater. The effects of two seawater acidification modes on the early development and survival of the sea urchin were studied. The results showed that both of the two seawater acidification modes reduced the floating rate of the sea urchin and the survival rate of the four Widespread floating larvae. Microscopic observations showed that both of the four Widespread sea urchin plateau larvae emerged under the two seawater acidification modes The absence of symmetry and the appearance of spicules, and the degree of symmetry loss increased with the decrease of seawater pH. The scanning electron microscopy revealed that both the surface and cross section of the spicules There are signs of corrosion, and the degree of dissolution with the sea water pH aggravated. The results suggest that acidification of seawater can influence the early development and survival of the sea urchin by reducing embryo floating rate, survival rate of larval larvae, destroying larval symmetry and structure of spicules.