论文部分内容阅读
目的:研究尖叶假龙胆不同提取部位对胰岛素抵抗H4ⅡE细胞糖消耗的影响。方法:采用CCK-8法评价H4ⅡE细胞活性;高浓度胰岛素(10-6mol/L)诱导培养H4ⅡE细胞36 h,建立新的胰岛素抵抗细胞模型。葡萄糖氧化酶法检测尖叶假龙胆不同提取部位对胰岛素抵抗H4ⅡE细胞糖消耗的影响。结果:尖叶假龙胆不同提取部位浓度大于150μg/m L时,对H4ⅡE细胞活性均有明显的抑制作用。尖叶假龙胆不同提取部位中乙酸乙酯提取部位对胰岛素抵抗H4ⅡE细胞葡萄糖消耗影响显著,大孔树脂95%提取部位与大孔树脂70%提取部位也不同程度地影响胰岛素抵抗H4ⅡE细胞葡萄糖消耗,大孔树脂30%提取部位效果不明显。结论:尖叶假龙胆乙酸乙酯提取部位改善胰岛素抵抗效果最佳,大孔树脂95%提取部位与大孔树脂70%提取部位也可不同程度地改善胰岛素抵抗作用。
OBJECTIVE: To study the effect of different extracts of Dictyostelium japonicum on glucose consumption in insulin resistance H4IIE cells. Methods: The activity of H4ⅡE cells was evaluated by CCK-8 assay. H4ⅡE cells were cultured for 36 h with high concentration of insulin (10-6 mol / L), and a new insulin resistance cell model was established. Effect of Different Extracts of Tipia pseudoacacia on Glucose Consumption in Insulin Resistance H4 Ⅱ E Cells Detected by Glucose Oxidase. Results: When the concentration of different extraction sites was more than 150μg / m L, the activity of H4ⅡE cells was significantly inhibited. Ethyl acetate extract of different extracts of A. nipponense had a significant effect on the glucose consumption of insulin resistance H4IIE cells. The fraction of macroporous resin (95%) and macroporous resin (70%) also affected the glucose consumption of insulin resistance H4IIE cells to varying degrees , Macroporous resin 30% extraction site effect is not obvious. CONCLUSION: Ethyl acetate extract of Rhizoma Imperatai has the best effect of improving insulin resistance. The extraction site of 95% macroporous resin and 70% of macroporous resin can also improve insulin resistance to some extent.