论文部分内容阅读
在８０～９０ｄ日龄Ｃ５７ＢＬ／６Ｊ小鼠上观察了１０～１０００μｇ／ｋｇｄ－１４种剂量ｒｈＩＬ－６对６．５Ｇｙγ线（１３２～１２７．７Ｒ／ｍｉｎ）照射后外周血细胞恢复的影响；观察时间持续至照射后３０天。结果表明：白细胞、红细胞和血小板计数的动态变化时间进程给药组和对照组相似，但总的趋势表明给药组各种血细胞计数的最低值高于对照组、各时间点的恢复情况均不同程度地优于对照组；除１０μｇ组外均有一部份时间点统计学差别显著，而５００和１０００μｇ剂量组效果尤为显著。血红蛋白含量和ＲＢＣ比积的变化与ＲＢＣ数一致。相关系数分析结果表明在一部分时间点上，外周血细胞数有明显的药量依赖性。照后２２天ＷＢＣ数、１４和１８天ＲＢＣ数以及１１和１８天ＰＬＴ数的剂量效应回归方程均具有统计学意义。本文结果表明：皮下注射ｒｈＩＬ－６剂量达到２００μｇ／ｋｇｄ－１以上连续４ｄ可减轻６．５Ｇｙ照射小鼠外周血细胞数下降程度并促进其恢复。其机制可能是通过ＩＬ－６对造血干、祖细胞增殖与分化的改善而起作用。
The effects of 10 ~ 1000μg / kg d-14 doses of rhIL-6 on the recovery of peripheral blood cells after 6.5Gyγ line irradiation (132 ~ 127.7R / min) were observed in 80-90 days old C57BL / 6J mice. Continued until 30 days after irradiation. The results showed that the time courses of leukocyte, erythrocyte and platelet counts were similar to those of the control group, but the general trend showed that the lowest value of the various blood cell counts in the administration group was higher than that in the control group, and the recovery was different at all time points The degree of superior to the control group; except for the 10μg group, there are statistically significant differences at some time points, while the 500 and 1000μg dose group effect is particularly significant. Changes in hemoglobin content and RBC product are consistent with RBC counts. Correlation coefficient analysis showed that at some time points, the number of peripheral blood cells was significantly dose-dependent. The regression equations of the dose-effect of WBC numbers, RBC counts on the 14th and 18th days and PLT numbers on the 11th and 18th days after 22 days were statistically significant. The results of this study show that: the subcutaneous injection of rhIL-6 dose of 200μg / kgd-1 more than 4d consecutive days can reduce the 6.5Gy irradiation mice peripheral blood cells decreased and promote its recovery. The mechanism may be through IL-6 hematopoietic stem and progenitor cell proliferation and differentiation play a role.