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研究杜仲叶浸提物对碳离子束辐照小鼠急性损伤的防护效应及其可能机制。小鼠灌胃不同剂量杜仲叶浸提物7 d后,6 Gy12C6+束全身均匀辐照,照射后12 h处死小鼠,取其肝、肺组织,分别用黄嘌呤氧化酶法和硫代巴比妥酸法(TBA法)检测组织中的SOD活性和MDA含量;用中性单细胞凝胶电泳技术(亦称彗星试验)检测组织细胞的DNA损伤;并用流式细胞术检测组织细胞的凋亡率。和单纯辐照组相比,预先灌服不同剂量的杜仲叶浸提物,能有效抑制重离子束辐照引起的小鼠肺和肝组织SOD活性降低和MDA含量提高;同时,还能使辐照小鼠肺和肝组织细胞的拖尾率、拖尾长度和细胞凋亡率显著降低,且有剂量效应关系。杜仲叶浸提物能显著减轻肝肺细胞DNA损伤和凋亡,推测这与其显著消除自由基作用有关。
Protective effects of Eucommia ulmoides leaf extract on acute injury induced by carbon ion beam in mice and its possible mechanism. Mice were gavaged with different doses of Eucommia extract 7 d after the 6 Gy12C6 + beam irradiation of the whole body 12 h after irradiation, mice were sacrificed, the liver and lung tissues were taken, respectively, with xanthine oxidase and thiobarbitol The TBA method was used to detect the activity of SOD and the content of MDA in the tissue. The DNA damage of tissue cells was detected by neutral single-cell gel electrophoresis (also called comet assay). The apoptosis of the cells was detected by flow cytometry rate. Compared with the pure irradiation group, the pretreatment with different doses of Eucommia leaf extracts could effectively inhibit the SOD activity and the MDA content in mice lung and liver tissue induced by heavy ion beam irradiation. At the same time, According to the mouse lung and liver cells tailing rate, tail length and apoptosis rate was significantly reduced, and the dose-response relationship. Eucommia leaf extract can significantly reduce DNA damage and apoptosis of liver cells, speculated that its significant role in the elimination of free radicals.