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目的:利用胸腺提取液(TE)探讨胸腺在重症肌无力(MG)发病中的作用。方法:取MG 儿童手术摘除胸腺制备 TE,用 MTT 法测定其对胸腺细胞及 PBMC 增殖的影响,用 ELISA 法测定 TE 中 SIL-2R 含量。结果:MG 患者 TE 对 PHA 活化的正常人的胸腺细胞增殖的抑制作用显著增强;对正常人 PBMC 增殖的影响,表现为高浓度抑制、低浓度促进的双向调节作用;TE中 SIL-2R 含量与正常人 TE 无显著差异。结论:MG 患者 TE 有显著异常,与 MG 发病有关,其免疫抑制活性增强的原因,似与 SIL-2R 无关。
Objective: To explore the role of thymus in the pathogenesis of myasthenia gravis (MG) by using thymus extract (TE). Methods: Thymus was excised from children undergoing MG surgery to determine the effect of TE on the proliferation of thymocytes and PBMC. The content of SIL-2R in TE was determined by ELISA. Results: The inhibitory effect of TE on proliferation of thymocytes in PHA-stimulated normal individuals was significantly enhanced. The effect of TE on the proliferation of normal human PBMCs was inhibited by high concentration and promoted by low concentration. The contents of SIL-2R in TE Normal TE no significant difference. Conclusion: There is a significant abnormality of TE in patients with MG, which is related to the pathogenesis of MG. The reason for its enhanced immunosuppressive activity seems to be unrelated to SIL-2R.