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探讨毛细管电泳对B族维生素的分离分析的最佳电泳条件 结果表明 :胶束电动力学毛细管色谱是分离分析B族维生素的一种有效的毛细管电泳操作模式 ;不同缓冲液及其pH对分离结果影响很大 ,10 0mmol/L硼酸钠缓冲液 (pH8 5)比较适合B族维生素的分离分析 ;毛细管温度、运行电压对分离也有较大影响 在 35cm× 50 μm未涂渍石英毛细管条件下 ,采用pH8 5,附加 50mmol/LSDS的 10 0mmol/L硼酸钠缓冲液 ,毛细管温度 2 0℃ ,运行电压 5～ 8kV ,能将水溶性维生素有效地分离 应用该电泳条件对“们泰其”麦绿宝进行维生素B6 、烟酸含量测定 ,结果表明该测定方法重复性较好 ,可以用于维生素的定性定量分析
The results of capillary electrophoresis showed that micellar electrokinetic capillary chromatography was an effective capillary electrophoresis mode of operation for the separation and analysis of B vitamins. The effect of different buffers and pH on the separation results Very large, 10 0mmol / L sodium borate buffer (pH8 5) is more suitable for the separation of B vitamins; capillary temperature, operating voltage has a greater impact on the separation of 35cm × 50μm uncoated quartz capillary conditions, the use of pH8 5, 50mmol / LSDS added 10 0mmol / L sodium borate buffer, capillary temperature 20 ℃, operating voltage 5 ~ 8kV, the water-soluble vitamins can be effectively separated by the electrophoresis conditions of the “Fateq” Mai Lvbao Vitamin B6, niacin content determination, the results show that the determination of reproducibility is good, can be used for qualitative and quantitative analysis of vitamins