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目的探讨干细胞因子(SCF)对高糖环境下豚鼠膀胱Cajal样间质细胞(ICCs-like)形态及电生理的影响。方法胶原酶消化法原代分离培养豚鼠膀胱ICCs样细胞。实验分组:正常对照组(葡萄糖浓度为5 mmol/L)、高糖组(葡萄糖浓度为15 mmol/L),培养基培养72 h后高糖组分为高糖对照组、SCF 50 ng/mL组、SCF 100 ng/mL组,继续培养24 h及72 h。激光共聚焦显微镜下测量细胞长度并获得每个时间点细胞内钙离子荧光强度值。结果随着培养时间延长,高糖对照组较正常对照组细胞长度明显缩短、钙离子荧光值显著降低(P<0.01)。SCF 50 ng/mL作用72 h后钙离子荧光值较高糖对照组增高(P<0.05)。SCF 100 ng/mL作用24 h后较高糖对照组细胞长度增长、钙离子荧光值增高(P<0.05),而作用72 h后细胞长度明显增长、钙离子荧光值显著增高(P<0.01)。结论外源性SCF干预后对高糖环境造成的ICCs样细胞形态及电生理功能异常有一定的改善或逆转作用。
Objective To investigate the effect of stem cell factor (SCF) on the morphology and electrophysiology of Cajal-like interstitial cells (ICCs-like) in guinea pig bladder under high glucose. Methods Primary isolation and culture of ICCs of guinea pig bladder with collagenase digestion method. The experimental groups were as follows: normal control group (glucose concentration 5 mmol / L) and high glucose group (glucose concentration 15 mmol / L) Group, SCF 100 ng / mL group, cultured for 24 h and 72 h. The length of cells was measured by laser confocal microscopy and the intracellular calcium fluorescence intensity was obtained at each time point. Results With the prolongation of culture time, the cell length of the high glucose control group was significantly shorter than that of the normal control group, and the calcium fluorescence value was significantly decreased (P <0.01). At 72 h SCF 50 ng / mL, the fluorescence value of calcium was higher than that of high glucose control group (P <0.05). After treated with SCF 100 ng / mL for 24 h, the cell length of the control group increased and the fluorescence of calcium increased (P <0.05), while the cell length increased significantly after 72 h (P <0.01) . Conclusion Exogenous SCF can improve or reverse the ICCs-like cell morphology and electrophysiological dysfunction induced by high glucose.