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目的建立兔眼脉络膜黑色素瘤动物模型,观察肿瘤的生长特性。设计实验研究。研究对象新西兰大白兔20只(20眼)。方法将培养的B16F10鼠皮肤黑色素瘤细胞悬液种植到C57BL/6黑鼠大腿内侧皮下,7～12天孵育成直径10mm团块后制成肿瘤碎片,种植到新西兰大白兔右眼脉络膜上腔,自种植之日起予环孢霉素A20mg/kg肌肉注射以抑制淋巴细胞免疫功能。每周行间接检眼镜、眼底照相、B超、彩色超声多普勒观察并记录兔眼底肿瘤大小、形状、眼部其他组织的改变以及肿瘤内部血液供应情况。观察6周后眼球摘除,行HE染色组织病理学检查。主要指标肿瘤最大基底径、高度、细胞类型、肿瘤内血管分布情况以及眼部其他组织肿瘤浸润情况。结果除1只(5%)兔因呕吐物窒息死亡外,余19只兔19眼(95%)肿瘤存活并迅速生长。其中1眼(5%)自种植4周开始肿瘤自行萎缩,第6周12眼(60%)肿瘤充满玻璃体腔。2周、3周、4周B超测量肿瘤平均最大基底径分别为3.1mm、6.1mm、12.2mm,平均高度分别为2.2mm、2.7mm、8.5mm。HE染色示肿瘤以梭形细胞为主,排列规则紧密,部分坏死,血管充盈。18眼(90%)均可见眼部其他组织受肿瘤细胞浸润,3眼(15%)肿瘤突破巩膜向球外蔓延。结论 B16F10鼠皮肤黑色素瘤细胞株兔眼脉络膜上腔种植后肿瘤迅速生长,模型建造成功率高。
Objective To establish an animal model of choroidal melanoma in rabbit eyes and observe the growth characteristics of the tumor. Design experiment research. Study New Zealand white rabbits 20 (20 eyes). Methods The cultured B16F10 murine melanoma cell suspension was subcutaneously implanted into the medial thigh of C57BL / 6 black mice. After 7 to 12 days of incubation, 10 mm diameter clumps were made into tumor fragments and implanted into the right eye choroidal cavity of New Zealand white rabbits. Cyclosporin A 20 mg / kg was intramuscularly injected to inhibit lymphocyte immune function from the day of planting. Weekly indirect ophthalmoscope, fundus photography, B ultrasound, color Doppler ultrasound and observe the size, shape of rabbit eyes, changes in other tissues of the eye and blood supply within the tumor. After 6 weeks of enucleation, histopathological examination was performed by HE staining. The main indicators of tumor maximum basal diameter, height, cell type, intravascular tumor distribution and other tissues of the eye tumor infiltration. Results Twenty-nine eyes (95%) of the remaining 19 rabbits survived and grew rapidly, except one (5%) of the rabbits died of suffocation of vomit. One of the eyes (5%) developed tumor atrophy on the 4th week after implantation, and 12 eyes (60%) of the 6th week filled the vitreous cavity. The average maximum basal diameters of tumors measured by B-mode ultrasonography at 2 weeks, 3 weeks and 4 weeks were 3.1mm, 6.1mm and 12.2mm, respectively. The average height were 2.2mm, 2.7mm and 8.5mm respectively. HE staining showed spindle-shaped tumor cells, arranged in close rules, some necrosis, vascular filling. Eighteen eyes (90%) showed that other tissues of the eye were infiltrated by tumor cells, and 3 eyes (15%) had a breakthrough in the sclera spreading out of the ball. Conclusion The tumor of B16F10 murine melanoma cell line grows rapidly after implantation in the suprachoroidal space of rabbit eyes. The success rate of model construction is high.