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为探讨血管活性肠肽（ＶＩＰ）对气道平滑肌增殖的影响及机制，我们以体外培养的犬气管平滑肌细胞为对象，以３Ｈ－ＴｄＲ掺入为指标，探讨了ＶＩＰ的作用，并分析了ＶＩＰ受体拮抗剂［４Ｃｌ－Ｄ－Ｐｈｅ６，Ｌｅｕ１７］ＶＩＰ，腺苷酸环化酶激活剂Ｆｏｒｓｋｏｌｉｎ，百日咳杆菌毒素（ＰＴｘ）和磷酯酶Ｃ抑制剂新霉素等对ＶＩＰ作用的影响。实验发现：ＶＩＰ对犬气道平滑肌细胞体外增殖有显著的抑制作用，且呈剂量依赖性。［４Ｃｌ－Ｄ－Ｐｈｅ６，Ｌｅｕ１７］－ＶＩＰ能完全阻断ＶＩＰ的作用，Ｆｏｒｓｋｏｌｉｎ则呈现出对ＶＩＰ的促进效应，ＰＴｘ和新霉素对ＶＩＰ作用无任何影响。上述结果提示：ＶＩＰ通过特异性受体在ＰＴｘ非敏感型Ｇ－蛋白介导下，激活ＡＣ，使平滑肌细胞内ｃＡＭＰ浓度增加，而发挥平滑肌细胞增生抑制效应
In order to investigate the effect of vasoactive intestinal peptide (VIP) on airway smooth muscle proliferation and its mechanism, we investigated the effect of VIP on 3H-TdR incorporation in vitro and in vitro. The effects of receptor antagonists [4Cl-D-Phe6, Leu17] VIP, adenylyl cyclase activators Forskolin, pertussis toxin (PTx) and phospholipase C inhibitor neomycin on VIP. The experiment found that: VIP can significantly inhibit the proliferation of canine airway smooth muscle cells in vitro and in a dose-dependent manner. [4Cl-D-Phe6, Leu17] -VIP completely blocked the effect of VIP, Forskolin showed a promoting effect on VIP, PTx and neomycin had no effect on VIP. The above results suggest that VIP activates AC through a specific receptor mediated by PTx non-sensitive G-protein, thereby increasing the cAMP concentration in smooth muscle cells and exerting an inhibitory effect on the proliferation of smooth muscle cells