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目的蜜蜂毒肽是蜜蜂粗毒中的主要物质。外周皮下注射蜜蜂毒肽可导致持续性自发痛和痛觉过敏。本研究旨在研究瞬时受体电势C(transient receptor potential canonical,TRPC)通道在蜜蜂毒肽诱致的初级感觉神经元活化中的介导作用。方法运用全细胞膜片钳和激光共聚焦测钙技术,检测TRPC通道抑制剂SKF-96365对蜜蜂毒肽诱致的急性分离大鼠背根神经节细胞胞内钙和内向电流升高的影响。结果电压钳记录的91个背根神经节细胞中,蜜蜂毒肽可诱发43.9%(40/91)的细胞产生内向电流,而不同浓度的SKF-96365(1,5,10μmol/L)均明显抑制了背根神经节细胞的内向电流,且呈剂量相关性。应用激光共聚焦钙成像技术记录的210个背根神经节细胞中,67.6%的细胞对蜜蜂毒肽敏感,产生胞内钙离子浓度的升高,而SKF-96365能抑制这种胞内钙浓度的升高,抑制率为46.5%。结论 SKF-96365能够抑制蜜蜂毒肽引起的背根神经节中小神经元的活化,提示TRPC通道介导了蜜蜂毒肽对初级感觉神经元的激活作用。
The purpose of bee poison peptide is the main material in bees venom. Peripheral subcutaneous injection of bee venom peptides can lead to persistent spontaneous pain and hyperalgesia. The purpose of this study was to investigate the role of transient receptor potential canonical (TRPC) channels in the activation of primary sensory neurons induced by bee venom peptides. Methods Whole cell patch clamp and laser confocal laser scanning calorimetry were used to detect the effect of TRPC channel inhibitor SKF-96365 on intracellular calcium and inward current in acellularly isolated rat dorsal root ganglion neurons. Results In the 91 dorsal root ganglion cells recorded by voltage clamp, inward current was induced in 43.9% (40/91) of cells by the bee venom peptide, while SKF-96365 (1, 5, 10μmol / L) Inhibit the inward current of DRG cells in a dose-dependent manner. Of the 210 dorsal root ganglion cells recorded using laser confocal calcium imaging, 67.6% of cells were sensitive to the bee venom peptide and produced an increase in intracellular calcium concentration, whereas SKF-96365 inhibited this intracellular calcium concentration Of the increase, the inhibition rate was 46.5%. Conclusion SKF-96365 can inhibit the activation of small neurons in the dorsal root ganglion induced by bee venom peptide, suggesting that TRPC channels mediate the activation of primary sensory neurons by the bee venom peptide.