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背景:研究表明,神经免疫调节肽α-黑素细胞刺激素(α-melanophore-stimulatinghormone,α-MSH)可通过抑制促炎因子释放、改善淋巴细胞活力等途径发挥其抗炎、抗菌、退热和免疫调节作用,在防治全身炎性反应综合征中可能有良好的应用前景。目的:研究α-MSH对缺血-再灌注后脑组织炎性细胞浸润及黏附分子(intercellularadhesionmolecule,ICAM-1)表达的影响。设计:随机双盲对照实验。单位:解放军第三军医大学西南医院急救部。材料:健康雄性Wistar大鼠60只,由第三军医大学实验动物中心提供。实验分3组,即假手术组、缺血组和α-MSH治疗组,每组根据缺血2h后再灌注6,12,24,48,72h5个时相点分为5组,每组各时相点4只。干预:制备大脑中动脉闭塞模型,在每个时间点取2只动物用免疫组织化学方法检测ICAM-1的表达情况。各时间点取2只动物用于检测髓过氧化物酶(myeloperoxidase,MPO)活性。在透射电镜下观察脑组织细胞超微结构。主要观察指标:ICAM-1阳性表达,MPO活性,细胞超微结构。结果:缺血再灌注后高倍镜下(×200)每平方毫米大鼠脑组织切片的I-CAM-1阳性细胞数在假手术组中稳定于(1.02±0.14)～(1.15±0.16)个/mm2;缺血组6h后ICAM-1开始上升,于12h达高峰,由(2.82±0.07)个/mm2增至(7.08±0.09)个/mm2,24～72h其表达水平
BACKGROUND: Studies have shown that α-melanophore-stimulating hormone (α-MSH) exerts its anti-inflammatory, antibacterial and antipyretic effects by inhibiting the release of proinflammatory cytokines and improving the activity of lymphocytes And immunomodulatory effects in the prevention and treatment of systemic inflammatory response syndrome may have a good prospect. Objective: To investigate the effect of α-MSH on the inflammatory cell infiltration and the expression of intercellular adhesion molecule (ICAM-1) in brain tissue after ischemia-reperfusion. Design: Randomized double-blind control experiment. Unit: First Military Medical University Southwest Hospital Emergency Department. MATERIALS: Sixty healthy male Wistar rats were provided by Experimental Animal Center of Third Military Medical University. The experimental groups were divided into 5 groups according to 5 hours after reperfusion: 6,12,24,48,72h after 2h of ischemia, sham operation group, ischemia group and α-MSH treatment group Time 4 points. Intervention: middle cerebral artery occlusion model was prepared. At each time point, 2 animals were used to detect the expression of ICAM-1 by immunohistochemical method. Two animals were taken at each time point to detect myeloperoxidase (MPO) activity. The ultrastructure of brain cells was observed under transmission electron microscope. MAIN OUTCOME MEASURES: ICAM-1 positive expression, MPO activity, cellular ultrastructure. RESULTS: The numbers of I-CAM-1 positive cells in the brain tissue sections under high magnification (× 200) per square millimeter after ischemia / reperfusion were stable in the sham operation group (1.02 ± 0.14) ~ (1.15 ± 0.16) / mm2. ICAM-1 began to rise in the ischemic group at 6h and peaked at 12h, from (2.82 ± 0.07) / mm2 to (7.08 ± 0.09) / mm2,24 to 72h