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采用石蜡切片,结合胰蛋白酶预处理,在研究大鼠出生前后海马结构谷氨酸样(Glutamate-Like.Glu-LI)和天门冬氨酸样(Aspartate-Like,Asp-LI)神经元的发育中取得了良好的效果.最大限度地降低了背景着色,使得细胞层次清晰易辨,镜下观察时不易出现视疲劳.不同年龄(或胎龄)的动物脑石蜡切片.为暴露抗原所需胰蛋白酶的浓度和消化时间不一样.一般年龄越小,所需胰蛋白酶浓度越小,消化时间也短.反之,所需浓度升高,消化时间延长.在适当的胰蛋白酶处理后,抗体稀释度也升高,一般I抗为1:10000～15000,Ⅱ、Ⅲ抗为1:500～1000.胰蛋白酶除了具有暴露抗原结合位点的功能外,还具有消除非特异性氧化物酶的能力.在石蜡切片中,H_2O_2和硼氢化钠的作用似乎无效.
Paraffin sections were used in combination with trypsin preconditioning to study the development of hippocampal glutamate-like glutamate (Glu-LI) and aspartate-like (Asp-LI) neurons in rats before and after birth Has achieved good results. Minimize the background coloring, making the cell level is clearly legible, microscopic observation is not prone to visual fatigue. Different age (or gestational age) animal brain paraffin section. To expose the antigen required for pancreatic Protease concentration and digestion time is not the same general age, the smaller the trypsin concentration required, the digestion time is short, on the contrary, the required concentration increased, digestion time extension in the appropriate trypsin treatment, antibody dilution Also increased, the general I anti-1: 10000 ~ 15000, Ⅱ, Ⅲ anti-1: 500 ~ 1000. In addition to trypsin exposure antigen binding sites, but also has the ability to eliminate non-specific oxidase in In paraffin sections, the effect of H 2 O 2 and sodium borohydride seems ineffective.