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目的探讨Ghrelin对正常小鼠空间学习记忆能力和海马神经元突触及生长激素促分泌素受体(GHS-R)表达的影响。方法 8周龄小鼠20只,随机分为实验组和对照组,分别腹腔注射Ghrelin和等量生理盐水,通过水迷宫实验检测小鼠的空间学习记忆能力,免疫组织化学实验检测海马Ghrelin受体GHS-R表达,并通过电子显微镜观察海马突触的超微结构变化。结果水迷宫隐匿平台实验中,第2、3、4天,实验组小鼠的逃避潜伏期与对照组相比明显缩短(P<0.05),跨越平台次数明显增加(P<0.05),海马CA3和齿状回区GHS-R免疫阳性产物表达明显增强(P<0.05),神经元突触数量明显增加(P<0.05)。结论 Ghrelin可能通过结合GHS-R,增加海马神经元突触数量,显著改善小鼠的空间学习记忆能力。
Objective To investigate the effects of Ghrelin on spatial learning and memory ability and the expression of synaptophysin and growth hormone secretagogue receptor (GHS-R) in hippocampal neurons of normal mice. Methods Twenty mice of 8 weeks old were randomly divided into experimental group and control group. Ghrelin and normal saline were intraperitoneally injected respectively. The spatial learning and memory abilities of mice were detected by water maze test. Immunohistochemistry was used to detect Ghrelin receptor GHS-R expression, and observed by electron microscopy ultrastructural changes in the hippocampal synapse. Results In the water maze concealed platform experiment, the escape latency of the mice in the experimental group was significantly shorter than that of the control group (P <0.05) on the 2nd, 3rd and 4th days, and significantly increased over the platform (P <0.05) The expression of GHS-R immunoreactive products in dentate gyrus was significantly increased (P <0.05), and the number of synapses in neurons was significantly increased (P <0.05). Conclusion Ghrelin may increase the number of synapses in hippocampal neurons by binding to GHS-R, and significantly improve the spatial learning and memory abilities of mice.